Human Sample Biorepository and Allocation Core

In collaboration with our ENT/Otolaryngology colleagues in Boston, we have developed a biorepository that provides both stored and fresh samples to collaborators for the advancement of biomedical research. Samples are obtained from participants undergoing elective sinus surgery and include sinus tissue, nasal polyp tissue, nasal fluid, nasal scrapings, nasal brushings, plasma/serum, peripheral blood mononuclear cells, and urine. Participants with the following medical diagnoses are included in the repository: aspirin-exacerbated respiratory disease (AERD), chronic rhinosinusitis with nasal polyposis (CRSwNP), chronic rhinosinusitis without nasal polyposis (CRSsNP), other sinus diseases, and healthy volunteers. All samples are linked to a clinical database as well. We have established protocols for all stages of cell processing and analysis, including histopathologic techniques as well as freezing, thawing, and culturing of isolated cells. Further, we regularly perform sinus tissue digestion and cell isolation through flow cytometric sorting, as well as single cell RNAseq from cell suspensions. Notable protocols have been published in peer-reviewed journals. These samples are stored within the Laidlaw Lab and are available to collaborators so that ongoing and future multidisciplinary research can be carried out.

Biorepository Director: Carolyn Baloh, MD

Biorepository Technician: Mabel Zawacki, BA

Research Coordinator: Laura Bailey, BA

The following are selected publications where fresh or stored samples were utilized:

Human intraepithelial mast cell differentiation and effector function are directed by TGF-β signaling.

Derakhshan T, Hollers E, Perniss A, Ryan T, McGill A, Hacker J, Bergmark RW, Bhattacharyya N, Lee SE, Maxfield AZ, Roditi RE, Bankova L, Buchheit KM, Laidlaw TM, Boyce JA, Dwyer DF. J Clin Invest. 2025 Jan 2;135(1):e174981. doi: 10.1172/JCI174981. PMID: 39744949; PMCID: PMC11684804.

Human nasal epithelial cells express IL-5Rα but not the co-receptor CSF2RB and do not signal to IL-5.

Sohail A, Cho L, Hacker J, Bergmark RW, Lee SE, Maxfield A, Roditi RE, Dwyer DF, Buchheit KM, Laidlaw TM. Allergy. 2024 Nov;79(11):3146-3150. doi: 10.1111/all.16218. Epub 2024 Jul 7. PMID: 38972002.

IL-4Rα signaling promotes barrier-altering oncostatin M and IL-6 production in aspirin-exacerbated respiratory disease.

Chen CC, Buchheit KM, Lee PY, Brodeur KE, Sohail A, Cho L, Baloh CH, Balestrieri B, Derakhshan T, Feng C, Boyce JA, Dwyer DF, Laidlaw TM. J Allergy Clin Immunol. 2024 Aug;154(2):458-467.e3. doi: 10.1016/j.jaci.2024.04.020. Epub 2024 May 3. PMID: 38704098; PMCID: PMC11305950.

TCF1-LEF1 co-expression identifies a multipotent progenitor cell (TH2-MPP) across human allergic diseases.

Kratchmarov R, Djeddi S, Dunlap G, He W, Jia X, Burk CM, Ryan T, McGill A, Allegretti JR, Kataru RP, Mehrara BJ, Taylor EM, Agarwal S, Bhattacharyya N, Bergmark RW, Maxfield AZ, Lee S, Roditi R, Dwyer DF, Boyce JA, Buchheit KM, Laidlaw TM, Shreffler WG, Rao DA, Gutierrez-Arcelus M, Brennan PJ. Nat Immunol. 2024 May;25(5):902-915. doi: 10.1038/s41590-024-01803-2. Epub 2024 Apr 8. PMID: 38589618.

A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation.

Ualiyeva S, Lemire E, Wong C, Perniss A, Boyd AA, Avilés EC, Minichetti DG, Maxfield A, Roditi R, Matsumoto I, Wang X, Deng W, Barrett NA, Buchheit KM, Laidlaw TM, Boyce JA, Bankova LG, Haber AL. Sci Immunol. 2024 Feb 2;9(92):eabq4341. doi: 10.1126/sciimmunol.abq4341. Epub 2024 Feb 2. PMID: 38306414; PMCID: PMC11127180.

Increased epithelial mTORC1 activity in chronic rhinosinusitis with nasal polyps.

Huang GX, Hallen NR, Lee M, Zheng K, Wang X, Mandanas MV, Djeddi S, Fernandez D, Hacker J, Ryan T, Bergmark RW, Bhattacharyya N, Lee S, Maxfield AZ, Roditi RE, Buchheit KM, Laidlaw TM, Gern JE, Hallstrand TS, Ray A, Wenzel SE, Boyce JA, Gutierrez-Arcelus M, Barrett NA.  bioRxiv [Preprint]. 2023 Oct 17:2023.10.13.562288. doi: 10.1101/2023.10.13.562288. PMID: 37904989; PMCID: PMC10614789.

Nasal polyp antibody-secreting cells display proliferation signature in aspirin-exacerbated respiratory disease.

Sohail A, Hacker J, Ryan T, McGill A, Bergmark R, Bhattacharyya N, Lee SE, Maxfield A, Roditi R, Julé AM, Griffith A, Lederer J, Laidlaw TM, Buchheit KM. J Allergy Clin Immunol. 2024 Feb;153(2):527-532. doi: 10.1016/j.jaci.2023.10.011. Epub 2023 Oct 28. PMID: 37898408; PMCID: PMC10922123.

Glucagon-like Peptide-1 Receptor Pathway Attenuates Platelet Activation in Aspirin-Exacerbated Respiratory Disease.

Foer D, Amin T, Nagai J, Tani Y, Feng C, Liu T, Newcomb DC, Lai J, Hayashi H, Snyder WE, McGill A, Lin A, Laidlaw TM, Niswender KD, Boyce JA, Cahill KN.  J Immunol. 2023 Dec 15;211(12):1806-1813. doi: 10.4049/jimmunol.2300102. PMID: 37870292; PMCID: PMC10842986.

Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single-cell RNA sequencing

Sohail A, Baloh CH, Hacker J, Cho L, Ryan T, Bergmark RW, Lee SE, Maxfield A, Roditi R, Dwyer DF, Buchheit KM, Laidlaw TM. Int Forum Allergy Rhinol. 2024 May;14(5):972-976. doi: 10.1002/alr.23275. Epub 2023 Sep 23. PMID: 37742089.

Type 2 inflammation drives an airway basal stem cell program through insulin receptor substrate signaling

Wang X, Hallen NR, Lee M, Samuchiwal S, Ye Q, Buchheit KM, Maxfield AZ, Roditi RE, Bergmark RW, Bhattacharyya N, Ryan T, Gakpo D, Raychaudhuri S, Dwyer D, Laidlaw TM, Boyce JA, Gutierrez-Arcelus M, Barrett NA.  J Allergy Clin Immunol. 2023 Jun;151(6):1536-1549. doi: 10.1016/j.jaci.2023.01.030. Epub 2023 Feb 17. PMID: 36804595; PMCID: PMC10784786.

Mediator production and severity of aspirin-induced respiratory reactions: Impact of sampling site and body mass index.

Cahill KN, Wu P, Milne GL, Amin T, Singer J, Murphy K, Lewis E, Gapko D, Boyce JA, Buchheit KM, Laidlaw TM. J Allergy Clin Immunol. 2022 Jul;150(1):170-177.e6. doi: 10.1016/j.jaci.2021.12.787. Epub 2022 Jan 11. PMID: 35026207; PMCID: PMC9271535.

Human airway mast cells proliferate and acquire distinct inflammation-driven phenotypes during type 2 inflammation

Dwyer DF, Ordovas-Montanes J, Allon SJ, Buchheit KM, Vukovic M, Derakhshan T, Feng C, Lai J, Hughes TK, Nyquist SK, Giannetti MP, Berger B, Bhattacharyya N, Roditi RE, Katz HR, Nawijn MC, Berg M, van den Berge M, Laidlaw TM, Shalek AK, Barrett NA, Boyce JA. Sci Immunol. 2021 Feb 26;6(56):eabb7221. doi: 10.1126/sciimmunol.abb7221. PMID: 33637594; PMCID: PMC8362933.

Influence of daily aspirin therapy on ACE2 expression and function-implications for SARS-CoV-2 and patients with aspirin-exacerbated respiratory disease.

Buchheit KM, Hacker JJ, Gakpo DH, Mullur J, Sohail A, Laidlaw TM.Clin Exp Allergy. 2021 Jul;51(7):968-971. doi: 10.1111/cea.13898. Epub 2021 May 26. PMID: 33987897; PMCID: PMC8239915.

SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues.

Ziegler CGK, Allon SJ, Nyquist SK, Mbano IM, Miao VN, Tzouanas CN, Cao Y, Yousif AS, Bals J, Hauser BM, Feldman J, Muus C, Wadsworth MH 2nd, Kazer SW, Hughes TK, Doran B, Gatter GJ, Vukovic M, Taliaferro F, Mead BE, Guo Z, Wang JP, Gras D, Plaisant M, Ansari M, Angelidis I, Adler H, Sucre JMS, Taylor CJ, Lin B, Waghray A, Mitsialis V, Dwyer DF, Buchheit KM, Boyce JA, Barrett NA, Laidlaw TM, Carroll SL, Colonna L, Tkachev V, Peterson CW, Yu A, Zheng HB, Gideon HP, Winchell CG, Lin PL, Bingle CD, Snapper SB, Kropski JA, Theis FJ, Schiller HB, Zaragosi LE, Barbry P, Leslie A, Kiem HP, Flynn JL, Fortune SM, Berger B, Finberg RW, Kean LS, Garber M, Schmidt AG, Lingwood D, Shalek AK, Ordovas-Montanes J; HCA Lung Biological Network. Electronic address: lung-network@humancellatlas.org; HCA Lung Biological Network. Cell. 2020 May 28;181(5):1016-1035.e19. doi: 10.1016/j.cell.2020.04.035. Epub 2020 Apr 27. PMID: 32413319; PMCID: PMC7252096.

IL-5Rα marks nasal polyp IgG4- and IgE-expressing cells in aspirin-exacerbated respiratory disease.

Buchheit KM, Dwyer DF, Ordovas-Montanes J, Katz HR, Lewis E, Vukovic M, Lai J, Bankova LG, Bhattacharyya N, Shalek AK, Barrett NA, Boyce JA, Laidlaw TM.  J Allergy Clin Immunol. 2020 Jun;145(6):1574-1584. doi: 10.1016/j.jaci.2020.02.035. Epub 2020 Mar 19. PMID: 32199912; PMCID: PMC7282948.

Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism

Liu T, Barrett NA, Kanaoka Y, Buchheit K, Laidlaw TM, Garofalo D, Lai J, Katz HR, Feng C, Boyce JA. Mucosal Immunol. 2019 May;12(3):679-690. doi: 10.1038/s41385-019-0134-8. Epub 2019 Jan 21. PMID: 30664709; PMCID: PMC6462243.

Allergic inflammatory memory in human respiratory epithelial progenitor cells

Ordovas-Montanes J, Dwyer DF, Nyquist SK, Buchheit KM, Vukovic M, Deb C, Wadsworth MH 2nd, Hughes TK, Kazer SW, Yoshimoto E, Cahill KN, Bhattacharyya N, Katz HR, Berger B, Laidlaw TM, Boyce JA, Barrett NA, Shalek AK. Nature. 2018 Aug;560(7720):649-654. doi: 10.1038/s41586-018-0449-8. Epub 2018 Aug 22. PMID: 30135581; PMCID: PMC6133715.

Samples are available for investigators at MGB.  To request samples please contact Laidlawsamples@bwh.harvard.edu.